Accurate identification of genetically modified animals is essential to research efficiency and to reducing the use of animals in experimental studies.
genotyping is generally carried out by analyzing DNA from tissues of young rodents; the most commonly used method for this purpose is polymerase chain reaction (PCR) analysis, as it requires a small amount of DNA.
when genotyping an animal is necessary, the method causing the least pain, distress and discomfort should be used
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When genotyping an animal is necessary, the method that causes the least pain, distress and discomfort should be used.
Within our research facilities, biologists and zootechnical staff have developed a hair sampling technique and made PCR DNA analysis more reliable, so as to reduce the contamination that previously penalized this method.
This approach, applied in a facility breeding transgenic animals for authorized research projects, offers the following benefits:
- A non-invasive, faster and more comfortable procedure, both for the animal and for the zootechnical staff, with less psychological impact.